[填空题]
The enzyme pepsin hydrolyses proteins ttiw 6/219ghjbdp qe1fo releasx 4p5 ,zj7tgwbe peptides in the stomach. A study was carried out to determine thbg wx5t,pj7 4ze effect of increasing substrate concentration on enzyme activity at a constant temperature of $30{\circ}C.
1.Describe the trends shown by the graph.
2. 1.Outline why it is important that temperature is kept constant in this experiment.
2.State one factor other than temperature that would need to be controlled in this experiment.
3.Suggest a method that can be used to maintain a constant temperature in this experiment.
3.Calculate the rate of reaction during the first 4 minutes when the enzyme pepsin is exposed to 5 mM of protein. Make sure you show your working.
4.Explain the observed results.
