[填空题]
The enzyme pepsin hydrolysesu: iia67 w+8k2e 3w tnofzzp2t proteins to release p7huv pq8-+l4ppyk 1nv eptides in the stomach. A study was carried out to determine the effect of i+8hv nq-p4y lp 71kpvuncreasing substrate concentration on enzyme activity at a constant temperature of $30{\circ}C.
1.Describe the trends shown by the graph.
2. 1.Outline why it is important that temperature is kept constant in this experiment.
2.State one factor other than temperature that would need to be controlled in this experiment.
3.Suggest a method that can be used to maintain a constant temperature in this experiment.
3.Calculate the rate of reaction during the first 4 minutes when the enzyme pepsin is exposed to 5 mM of protein. Make sure you show your working.
4.Explain the observed results.
